Regulatory

Part:BBa_K1440003:Design

Designed by: Xuanye Cao   Group: iGEM14_Fudan   (2014-11-01)


PTRE promoter>OSKM


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 435
    Illegal PstI site found at 846
    Illegal PstI site found at 2196
    Illegal PstI site found at 3041
    Illegal PstI site found at 4305
    Illegal PstI site found at 4722
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 435
    Illegal NheI site found at 1500
    Illegal PstI site found at 846
    Illegal PstI site found at 2196
    Illegal PstI site found at 3041
    Illegal PstI site found at 4305
    Illegal PstI site found at 4722
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 435
    Illegal BamHI site found at 530
    Illegal XhoI site found at 2604
    Illegal XhoI site found at 4059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 435
    Illegal PstI site found at 846
    Illegal PstI site found at 2196
    Illegal PstI site found at 3041
    Illegal PstI site found at 4305
    Illegal PstI site found at 4722
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 435
    Illegal PstI site found at 846
    Illegal PstI site found at 2196
    Illegal PstI site found at 3041
    Illegal PstI site found at 4305
    Illegal PstI site found at 4722
    Illegal NgoMIV site found at 2022
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4622
    Illegal BsaI.rc site found at 1092
    Illegal BsaI.rc site found at 1158
    Illegal BsaI.rc site found at 1833
    Illegal BsaI.rc site found at 2886
    Illegal BsaI.rc site found at 4554
    Illegal SapI.rc site found at 4947


Design Notes

none


Source

yes, Oct4,Sox2,Klf4 and cMyc come from mouse genome.

References